Best ICSI Center in Mumbai

For male infertile patients, intracytoplasmic sperm injections (ICSI) are used. It is done as part of the process known as in vitro fertilization (IVF). The sperm is directly injected into the egg during this medical procedure. For certain men, sperm extraction surgery may be necessary beforehand. Many couples experience difficulties conceiving a child. Everyone knows that sperm-related factors contribute to infertility.

ICSI treatment might be recommended by the doctor if :

• The sperms are abnormally shapes
• If you have undergone IVF previously and no positive results have been found
• You need sperm to be collected surgically from the testicles or epididymis (a narrow tube inside the scrotum where sperm are stored and matured); for example, because you have had a vasectomy, or you have a blockage that prevents sperm from reaching the ejaculate, caused by disease, injury, or a genetic condition, or because you have an extremely low sperm count
• You’re using frozen sperm in your treatment which isn’t of the highest quality, especially if it was stored because your fertility was under threat, or following surgical sperm retrieval.
• You’re having embryo testing for a genetic condition, and sperm sticking to the outside of the eggs would interfere with the results

How successful is one cycle of ICSI?

ICSI is a very effective tool for facilitating fertilization between the sperm and the egg. A successful pregnancy is still influenced by a number of other factors, such as the woman’s age and whether or not she has experienced problems with fertility in the past. This is similar to IVF. Each woman’s ICSI success rate is unique. Visits to reputable ICSI Clinics are necessary.

Dr L H Hiranandani hospital is ranked among the best ICSI Clinic in Mumbai

ICSI Treatment – How Does It Work?

ICSI is generally performed following a transvaginal oocyte retrieval procedure to extract one or several oocytes from a woman. In ICSI IVF, the male partner or a donor provides a sperm sample on the same day when the eggs are collected.[20] The sample is checked in the lab, and if no sperm is present, doctors will extract sperm from the epididymis or testicle. The extraction of sperm from epididymis is also known as percutaneous epididymal sperm aspiration (PESA) and the extraction of sperm from the testicle is also known as testicular sperm aspiration (TESA). Depending on the total amount of spermatozoa in the semen sample, either low or high, it can be just washed or capacitated via swim-up or gradients, respectively.

The procedure is done under a microscope using multiple micromanipulation devices (micromanipulator, microinjectors, and micropipettes). A holding pipette stabilizes the mature oocyte with gentle suction applied by a microinjector. From the opposite side a thin, hollow glass micropipette is used to collect a single sperm, having immobilized it by cutting its tail with the point of the micropipette. The oocyte is pierced through the oolemma and the sperm is directed into the inner part of the oocyte (cytoplasm). The sperm is then released into the oocyte. The pictured oocyte has an extruded polar body at about 12 o’clock indicating its maturity. The polar body is positioned at the 12 or 6 o’clock position, to ensure that the inserted micropipette does not disrupt the spindle inside the egg. After the procedure, the oocyte will be placed into a cell culture and checked on the following day for signs of fertilization.

In contrast, in natural fertilization sperm compete and when the first sperm penetrates the oolemma, the oolemma hardens to block the entry of any other sperm. Concern has been raised that in ICSI this sperm selection process is bypassed and the sperm is selected by the embryologist without any specific testing. However, in mid-2006 the FDA cleared a device that allows embryologists to select mature sperm for ICSI based on sperm binding to hyaluronan, the main constituent of the gel layer (cumulus oophorus) surrounding the oocyte. The device provides microscopic droplets of hyaluronan hydrogel attached to the culture dish. The embryologist places the prepared sperm on the microdot and selects and captures sperm that bind to the dot. Basic research on the maturation of sperm shows that hyaluronan-binding sperm are more mature and show fewer DNA strand breaks and significantly lower levels of aneuploidy than the sperm population from which they were selected. A brand name for one such sperm selection device is PICSI. A recent clinical trial showed a sharp reduction in miscarriage with embryos derived from PICSI sperm selection

Our ICSI Treatment in Mumbai

IVF stands for In Vitro Fertilization. It is the process by which a woman’s eggs are collected and then fertilized outside her womb in the lab. ICSI (Intracytoplasmic Sperm Injection) has proved to be very useful in couples who have failed to accomplish fertilization or had very poor fertilization following standard IVF treatment & in couples where the male partner has abnormal sperm parameters (e.g. low count, poor motility, a high percentage of abnormal sperms). The IVF / ICSI treatment does not require overnight hospitalization. We are the top ICSI center in Mumbai